prccmv myc plk1 t210d (Addgene inc)

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Addgene inc prccmv myc plk1 t210d
Prccmv Myc Plk1 T210d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prccmv+myc+plk1/pRcCMV+myc-Plk1+T210D+(Nigg+LA183)+(Plasmid+%2341158)/pmc12301659-241-11-15
Average 93 stars, based on 1 article reviews

prccmv myc plk1 t210d - by Bioz Stars, 2026-09

93/100 stars

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Expressing:

Article Title: Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila.
Article Snippet: .. The Vrk3 mammalian expression construct was generated by recombining pDONR223-VRK3 (Addgene 2368767) into pDEST-CMV-3xFLAG-EGFP (Addgene 12284568); pRcCMV-Myc-Plk1 was used without modification (Addgene 4116069). ..

Article Title: Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila
Article Snippet: .. The Vrk3 mammalian expression construct was generated by recombining pDONR223-VRK3 (Addgene 23687 ) into pDEST-CMV-3xFLAG-EGFP (Addgene 122845 ); pRcCMV-Myc-Plk1 was used without modification (Addgene 41160 ). .. Antibodies used were α-Mef2 (1:1000, gift from R. Cripps), α-Tropomyosin (1:600, Abcam, MAC141), α-PLK1-phospho-T210 (1:100, Abcam, ab39068), α-GFP (1:600, Torrey Pines Biolabs, TP-401), α-GFP (1:300, Aves Labs, GFP-1020), α-dsRed (1:300, Takara, 632392), α-γ-tubulin (1:300, Sigma, T5326), and α-βgal (1:100, Promega, Z3781).

Article Title: Spatiotemporal expression of regulatory kinases directs the transition from mitotic growth to cellular morphogenesis
Article Snippet: Plasmids for expressing tagged proteins in S2 cells were 269 generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and 270 recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF). .. The Vrk3 mammalian expression construct was 274 generated by recombining pDONR223-VRK3 [Addgene 23687, [63]] into pDEST-CMV-3xFLAG-275 14 EGFP [Addgene 122845, [64]]; pRcCMV-Myc-Plk1 was used without modification [Addgene 276 41160, [65]]. ..

Article Title: Spatiotemporal expression of regulatory kinases directs the transition from mitotic growth to cellular morphogenesis
Article Snippet: .. The Vrk3 mammalian expression construct was generated by recombining pDONR223-VRK3 [Addgene 23687, [ ]] into pDEST-CMV-3xFLAG-EGFP [Addgene 122845, [ ]]; pRcCMV-Myc-Plk1 was used without modification [Addgene 41160, [ ]]. .. Antibodies used include α-Mef2 (1:1000, gift from R. Cripps), α-Tropomyosin (1:600, Abcam, MAC141), α-PLK1-phospho-T210 (1:100, Abcam, ab39068), α-GFP (1:600, Torrey Pines Biolabs, TP-401), and α-βgal (1:100, Promega, Z3781).

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Recombinant:

Article Title: An order-to-disorder structural switch activates the FoxM1 transcription factor
Article Snippet: Recombinant DNA reagent , pET-14B , Addgene , , Engineered to contain TEV protease site. .. Recombinant DNA reagent , pRcCMV myc-Plk1 , Addgene; PMID: 7962193 , , Erich Nigg. .. Recombinant DNA reagent , pGL3-6DB , PMID: 22094256 , , Peter Sicinski (Dana Farber Cancer Institute).

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